Herbal Medicine: Open Access

Crop improvement and tolerant to Yam Virus Yam (YVY) through agrobacteruim mediated gene transfer to bring positivity result in specific gene mutation of yam (dioscorea rotundata) for crop adaptability

Inheritable metamorphosis of yams asexually propagated has been extensively used as a tool for yams enhancement and as a vital part of the development of functional genomics coffers, there has been no report of any being Agrobacterium- intermediated metamorphosis of yam (Dioscoreaspp.) with substantiation of stable integration of YVy. Dioscorea is an important crop in the tropics and subtropics furnishing food security and income to million people. Still, yam product remains constrained by adding situations of field and storehouse pests and conditions. A major constraint to the development of biotechnological approaches for yam enhancement has been the lack of an effective and robust metamorphosis and rejuvenescence system. In this study, we developed an Agrobacterium- intermediated metamorphosis of Dioscorea rotundata using side kids as explants. Two cultivars of D. rotundata were converted using Agrobacterium tumefaciens harboring the binary vectors containing selectable marker and journalist genes. After selection with applicable attention of antibiotic, shoots were developed on shoot induction and extension medium. The elongated antibiotic-resistant shoots were latterly embedded on medium supplemented with selection agent. Successful metamorphosis was verified by polymerase chain response, Southern spot analysis, and journalist genes assay. Expression of YVy gene in transgenic shops was also vindicated by rear recap polymerase chain response analysis. Transformation effectiveness varied from7.4 to17.3 depending on the cultivars, selectable marker genes, and the Agrobacterium strain used for metamorphosis. It took 4-6 months from Agro-infection to rejuvenescence of complete transgenic factory. Then we report an effective, fast and reproducible protocol for Agrobacterium- intermediated metamorphosis ofD. rotundata using side kids as explants, which provides a useful platform for unborn inheritable.

Author(s): Moses Mathenge

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