Background: About, five percent of the population in the world has been clinically determined to have diabetes mellitus and considered as one of the principal reasons for death. The present research designed to assess the impact of Rhizophora mucronata leaves ethanolic extract (EtOH-Et) and its dichloromethane (DCM) and aqueous (Aq) fractions in diabetic rats.
Methods: Extract and fractions of R. mucronata were determined qualitative and quantitative methods. Different dose (50, 100 and 200 mg/kg of body weight) of extract and fractions were administered intraperitoneally (I.P.) to the normal glycemic rats and their hypoglycemic effect determined for 24 h. Type 1 diabetes mellitus (T1DM) was induced by single injection of streptozotocin (STZ; 60 mg/ kg; I.P.), and type 2 diabetes mellitus (T2DM) was induced by STZ (60 mg/kg; I.P.), after 15 min nicotinamide (120 mg/kg; I.P.). The optimum dose of 100 mg/kg body weight of extract and fractions was administrated to the rats orally for 14 days. The blood glucose level estimated on 0th, 7th, and 14th day. The level of serum cholesterol, triglycerides, and lipoprotein cholesterol were also evaluated on the 14th day.
Results: The phytochemical results indicated alkaloids, flavonoids, and terpenoids present in the concentrates of R. mucronata. The content of alkaloids in DCM-F was present in high amount compared with ethanolic extract and an aqueous fraction. All the extract exhibited as non-toxic nature. DCM-F treated rats significantly reduced in blood glucose level (P<0.01), serum cholesterol (P<0.05) and triglycerides (P<0.05) levels whereas HDL-C level was found to be increased (P<0.05) as compared with the diabetic control of T1DM and T2DM.
Conclusion: DCM-F of R. mucronata act as effective anti-hyperglycaemic and antihyperlipidemic agent in insulin dependent and non-insulin dependent diabetic rats.
Gurudeeban S,Satyavani Kaliamurthi and Ramanathan Thirugnanasambandam
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